One hepatocyte, alive: glycogen fills and empties on a measured feeding cycle, CYP3A4 clears substrate, bile leaves at the canaliculus, and a hepatectomy rebuilds the cell. The budget bar below is the CYP3A4 intrinsic clearance.
CYP3A4 CLint per gram liver
271 uL/min/g 24.7 to 2,148 uL/min/g across the sourced ranges. No sourced capacity for this quantity, so the bar places this cell between the smallest and the largest the sourced ranges allow.
Intrinsic hepatic clearance per gram of liver; the product the hepatocyte population delivers to a pharmacokinetic model. Sustained by CYP3A4 enzyme density and substrate affinity.
source 1source 2,
CLint per million cells
2.73 uL/min
0.333 to 16.4 uL/min propagated
CLint per gram liver
271 uL/min/g
24.7 to 2,148 uL/min/g propagated
Withheld
Space of Disse width
PMID 3088829 (Sztark 1986) reports volume fractions for endothelial cells (8.2%) and perisinusoidal cells / Ito cells (4.7%) of sinusoid volume — not the width of the extracellular Disse space in um. No abstract in the sweep states the Disse space width in um for human liver. Range and default were invented from a misread fraction and an uncited sinusoid diameter. Requires a human EM morphometry abstract with the measured width before shipping. Sheet standoff position in the drawing is illustrative and marked as such.
Unbound plasma fraction
Unbound fraction in plasma is substrate-specific and cannot be given a cell-level default from a verified hepatocyte abstract; include fu when predicting in vivo clearance for a named drug.